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R&D Systems csf1

CSF1R ligands boost microglial proliferation in serum-free primary microglial culture. (A) Schematic of immunopanning for the isolation and culture of murine microglia. I. V., in vitro . Schematic created in BioRender by Ho, M. 2025. https://BioRender.com/zvx72w4 . This figure was sublicensed under CC-BY 4.0 terms. (B) A plot of the percentage of total Cd11b + microglia that were KI67 + after a 72-h treatment of 10 ng/ml of each predicted signalling factor in microglial base media. (C) Representative images of proliferative microglia (CD11b + KI67 + ) at 1000 ng/ml. Scale bars: 100 µm. (D) A plot of the mean percentage of microglia that are proliferative at tenfold increasing concentrations of <t>CSF1</t> or IL34. (E) A plot of the mean percentage of viable microglial sustained by tenfold increasing concentrations of CSF1 or IL34. BM here indicates a microglial base media control supplemented with 2 ng/ml TGFβ2; GM indicates microglial growth media control containing both 2 ng/ml TGFβ2 and 10 ng/ml CSF1. (F) A plot of the percentage of live microglia sustained by BM containing either 1 ng/ml or 10 ng/ml CSF1. Bars represent mean±s.e.m.; B: n =2, D-F: n =3, independent microglial cultures with treatments in triplicate or quadruplicate. **** P <0.0001 (one-way ANOVA, Tukey post-hoc). ns, not significant.

Csf1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+m+csf+elisa+kit/Mouse+M-CSF+Quantikine+ELISA+Kit/pmc12188242-241-6-7
Average 93 stars, based on 59 article reviews

csf1 - by Bioz Stars, 2026-10

93/100 stars

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1) Product Images from "CSF1R ligands promote microglial proliferation but are not the sole regulators of developmental microglial proliferation"

Article Title: CSF1R ligands promote microglial proliferation but are not the sole regulators of developmental microglial proliferation

Journal: Development (Cambridge, England)

doi: 10.1242/dev.204610

Figure Legend Snippet: CSF1R ligands boost microglial proliferation in serum-free primary microglial culture. (A) Schematic of immunopanning for the isolation and culture of murine microglia. I. V., in vitro . Schematic created in BioRender by Ho, M. 2025. https://BioRender.com/zvx72w4 . This figure was sublicensed under CC-BY 4.0 terms. (B) A plot of the percentage of total Cd11b + microglia that were KI67 + after a 72-h treatment of 10 ng/ml of each predicted signalling factor in microglial base media. (C) Representative images of proliferative microglia (CD11b + KI67 + ) at 1000 ng/ml. Scale bars: 100 µm. (D) A plot of the mean percentage of microglia that are proliferative at tenfold increasing concentrations of CSF1 or IL34. (E) A plot of the mean percentage of viable microglial sustained by tenfold increasing concentrations of CSF1 or IL34. BM here indicates a microglial base media control supplemented with 2 ng/ml TGFβ2; GM indicates microglial growth media control containing both 2 ng/ml TGFβ2 and 10 ng/ml CSF1. (F) A plot of the percentage of live microglia sustained by BM containing either 1 ng/ml or 10 ng/ml CSF1. Bars represent mean±s.e.m.; B: n =2, D-F: n =3, independent microglial cultures with treatments in triplicate or quadruplicate. **** P <0.0001 (one-way ANOVA, Tukey post-hoc). ns, not significant.

Techniques Used: Isolation, In Vitro, Control

CSF1 and IL34 protein increase throughout development and display distinct spatiotemporal transcriptional expression patterns. (A,B) IL34 (A) and CSF1 (B) protein levels in whole-brain homogenates as measured by ELISA. (C) Representative brain microscopy image with regions of interest outlined in red. Scale bar: 100 µm. (D,F) Representative images of Il34 transcript visualized with RNAscope™ in CA1 (D) and somatosensory cortex (F). Scale bars: 40 µm. (E,G) Plots of the mean percentage of total cells of CA1 (E) and somatosensory cortex (G) that are Il34 + across development. (H,J) Representative images of Csf1 transcript visualized with RNAscope™ in CA1 (H) and somatosensory cortex (J). Scale bars: 40 µm. (I,K) Plots of the mean percentage of total cells of CA1 (I) and somatosensory cortex (K) that are Csf1 + across development. (L) Density maps depicting the spatiotemporal expression patterns of Il34 and Csf1 transcripts in RNAscope™ across development. Warmer colours indicate greater, while cooler colours indicate lower, transcript density. Bars represent mean±s.e.m.; A,B: n =5 mice per time point; E: n =3-5 mice per time point, G: n =4-5 mice per time point, I,K: n =2-3 mice per time point. * P <0.05, **** P <0.0001 (one-way ANOVA, Tukey post-hoc).

Figure Legend Snippet: CSF1 and IL34 protein increase throughout development and display distinct spatiotemporal transcriptional expression patterns. (A,B) IL34 (A) and CSF1 (B) protein levels in whole-brain homogenates as measured by ELISA. (C) Representative brain microscopy image with regions of interest outlined in red. Scale bar: 100 µm. (D,F) Representative images of Il34 transcript visualized with RNAscope™ in CA1 (D) and somatosensory cortex (F). Scale bars: 40 µm. (E,G) Plots of the mean percentage of total cells of CA1 (E) and somatosensory cortex (G) that are Il34 + across development. (H,J) Representative images of Csf1 transcript visualized with RNAscope™ in CA1 (H) and somatosensory cortex (J). Scale bars: 40 µm. (I,K) Plots of the mean percentage of total cells of CA1 (I) and somatosensory cortex (K) that are Csf1 + across development. (L) Density maps depicting the spatiotemporal expression patterns of Il34 and Csf1 transcripts in RNAscope™ across development. Warmer colours indicate greater, while cooler colours indicate lower, transcript density. Bars represent mean±s.e.m.; A,B: n =5 mice per time point; E: n =3-5 mice per time point, G: n =4-5 mice per time point, I,K: n =2-3 mice per time point. * P <0.05, **** P <0.0001 (one-way ANOVA, Tukey post-hoc).

Techniques Used: Expressing, Enzyme-linked Immunosorbent Assay, Microscopy, RNAscope

CSF1R signalling is largely restricted to microglia and border-associated macrophages in the P7 hippocampus. (A) UMAP plot depicting the cellular populations of the P7 hippocampus. Dots represent individual cells clustered based on transcriptional similarity, while colours indicate distinct cell lineages or cellular states based on gene expression patterns. (B) UMAP plot depicting expression of Csf1r in the P7 hippocampus. (C) UMAP plot depicting expression of Csf1 in the P7 hippocampus. (D) UMAP plot depicting expression of Il34 in the P7 hippocampus. (E) Dot plot depicting the cell population expression levels of Csf1r , Csf1 and Il34 in the P7 hippocampus. (F) CellChat plot predicting the dominant sender populations that secrete CSF1 and IL34 and dominant receiver populations that receive signalling via CSF1R in the P7 hippocampus. (G) Violin plot depicting the expression of microglial Csf1r across the mouse lifespan. (H) UMAP plot depicting inhibitory neurons, excitatory neurons and non-neuronal cells of the adult hippocampus. Dots represent individual cells clustered based on transcriptional similarity, while colours indicate distinct cell lineages or cellular states based on gene expression patterns. (I) UMAP plot depicting expression of Il34 in the adult hippocampus. (J) Violin plot comparing the expression level of Il34 between inhibitory neurons, excitatory neurons and non-neuronal cells of the adult hippocampus. Datasets for A-F: ; G: ; H-J: . G,J: **** P <0.0001 (one-way ANOVA, Tukey post-hoc).

Figure Legend Snippet: CSF1R signalling is largely restricted to microglia and border-associated macrophages in the P7 hippocampus. (A) UMAP plot depicting the cellular populations of the P7 hippocampus. Dots represent individual cells clustered based on transcriptional similarity, while colours indicate distinct cell lineages or cellular states based on gene expression patterns. (B) UMAP plot depicting expression of Csf1r in the P7 hippocampus. (C) UMAP plot depicting expression of Csf1 in the P7 hippocampus. (D) UMAP plot depicting expression of Il34 in the P7 hippocampus. (E) Dot plot depicting the cell population expression levels of Csf1r , Csf1 and Il34 in the P7 hippocampus. (F) CellChat plot predicting the dominant sender populations that secrete CSF1 and IL34 and dominant receiver populations that receive signalling via CSF1R in the P7 hippocampus. (G) Violin plot depicting the expression of microglial Csf1r across the mouse lifespan. (H) UMAP plot depicting inhibitory neurons, excitatory neurons and non-neuronal cells of the adult hippocampus. Dots represent individual cells clustered based on transcriptional similarity, while colours indicate distinct cell lineages or cellular states based on gene expression patterns. (I) UMAP plot depicting expression of Il34 in the adult hippocampus. (J) Violin plot comparing the expression level of Il34 between inhibitory neurons, excitatory neurons and non-neuronal cells of the adult hippocampus. Datasets for A-F: ; G: ; H-J: . G,J: **** P <0.0001 (one-way ANOVA, Tukey post-hoc).

Techniques Used: Gene Expression, Expressing

Unaltered Il34 and Csf1 levels of IL1α and IL1β knockout mice are insufficient to explain aberrant developmental microglial proliferation. (A) Representative whole brain microscopy image with analysed regions of interest outlined in red. (B) Representative images of IBA1 + microglia (red) and KI67 + proliferative cells (white) in the P10 hippocampus and cortex of IL1α and IL1β knockout mice. Scale bars: 40 µm. (C,D) Plots of the mean percentage of hippocampal microglia (C) and somatosensory cortex microglia (D) that are proliferating in wild-type (WT), IL1α and IL1β knockout mice across development. (E,F) Plots of mean hippocampal microglial densities (E) and mean somatosensory cortex microglial densities (F) in WT, IL1α and IL1β knockout mice. (G,H) Plots of the mean percentage of total cells of CA1 that are Il34 + (G) or Csf1 + (H) at P10 in WT, IL1α and IL1β knockout mice. (I,J) Plots of the mean percentage of total cells of somatosensory cortex that are Il34 + (I) or Csf1 + (J) at P10 in WT, IL1α and IL1β knockout mice. Bars represent mean±s.e.m.; C-F: n =6-8 mice per time point; G,I: n =5 mice per genotype; H,J: n =3-5 mice per genotype. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (C-F: two-way ANOVA, Tukey post-hoc; G-J: one-way ANOVA, Tukey post-hoc).

Figure Legend Snippet: Unaltered Il34 and Csf1 levels of IL1α and IL1β knockout mice are insufficient to explain aberrant developmental microglial proliferation. (A) Representative whole brain microscopy image with analysed regions of interest outlined in red. (B) Representative images of IBA1 + microglia (red) and KI67 + proliferative cells (white) in the P10 hippocampus and cortex of IL1α and IL1β knockout mice. Scale bars: 40 µm. (C,D) Plots of the mean percentage of hippocampal microglia (C) and somatosensory cortex microglia (D) that are proliferating in wild-type (WT), IL1α and IL1β knockout mice across development. (E,F) Plots of mean hippocampal microglial densities (E) and mean somatosensory cortex microglial densities (F) in WT, IL1α and IL1β knockout mice. (G,H) Plots of the mean percentage of total cells of CA1 that are Il34 + (G) or Csf1 + (H) at P10 in WT, IL1α and IL1β knockout mice. (I,J) Plots of the mean percentage of total cells of somatosensory cortex that are Il34 + (I) or Csf1 + (J) at P10 in WT, IL1α and IL1β knockout mice. Bars represent mean±s.e.m.; C-F: n =6-8 mice per time point; G,I: n =5 mice per genotype; H,J: n =3-5 mice per genotype. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (C-F: two-way ANOVA, Tukey post-hoc; G-J: one-way ANOVA, Tukey post-hoc).

Techniques Used: Knock-Out, Microscopy

Enzyme-linked Immunosorbent Assay:

Article Title: ERK signaling regulates macrophage colony-stimulating factor expression induced by titanium particles in MC3T3.E1 murine calvarial preosteoblastic cells.
Article Snippet: Periprosthetic osteolysis poses a significant clinical problem for patients who have undergone total joint arthroplastic surgeries.. It has been widely recognized that there is a strong correlation between wear particles from orthopedic implants and osteolysis.. However, the molecular mechanism underlying osteolysis still remains unclear.

Article Title: Osteoclasts are dispensable for hematopoietic stem cell maintenance and mobilization
Article Snippet: Stained cells were analyzed on a FACS Aria2 machine (BD). .. Serum M-CSF levels were assayed using the Mouse M-CSF ELISA kit (R&D Systems), according to the manufacturer’s instructions. .. Frozen sections of op/op mice femoral bone that had not been decalcified were stained with goat anti–c-Kit antibody (AF1356; R&D Systems), followed by Alexa Fluor 488–conjugated donkey anti–goat antibody (Invitrogen) to detect HSCs and with TOTO3 (Invitrogen) to detect nuclei.

Image Search Results

Journal: Development (Cambridge, England)

Article Title: CSF1R ligands promote microglial proliferation but are not the sole regulators of developmental microglial proliferation

doi: 10.1242/dev.204610

Figure Lengend Snippet: CSF1R ligands boost microglial proliferation in serum-free primary microglial culture. (A) Schematic of immunopanning for the isolation and culture of murine microglia. I. V., in vitro . Schematic created in BioRender by Ho, M. 2025. https://BioRender.com/zvx72w4 . This figure was sublicensed under CC-BY 4.0 terms. (B) A plot of the percentage of total Cd11b + microglia that were KI67 + after a 72-h treatment of 10 ng/ml of each predicted signalling factor in microglial base media. (C) Representative images of proliferative microglia (CD11b + KI67 + ) at 1000 ng/ml. Scale bars: 100 µm. (D) A plot of the mean percentage of microglia that are proliferative at tenfold increasing concentrations of CSF1 or IL34. (E) A plot of the mean percentage of viable microglial sustained by tenfold increasing concentrations of CSF1 or IL34. BM here indicates a microglial base media control supplemented with 2 ng/ml TGFβ2; GM indicates microglial growth media control containing both 2 ng/ml TGFβ2 and 10 ng/ml CSF1. (F) A plot of the percentage of live microglia sustained by BM containing either 1 ng/ml or 10 ng/ml CSF1. Bars represent mean±s.e.m.; B: n =2, D-F: n =3, independent microglial cultures with treatments in triplicate or quadruplicate. **** P <0.0001 (one-way ANOVA, Tukey post-hoc). ns, not significant.

Article Snippet: Both IL34 (R&D Systems, M3400) and CSF1 (R&D Systems, MMC00B) ELISAs were conducted as per the manufacturer's instructions.

Techniques: Isolation, In Vitro, Control

Journal: Development (Cambridge, England)

Article Title: CSF1R ligands promote microglial proliferation but are not the sole regulators of developmental microglial proliferation

doi: 10.1242/dev.204610

Figure Lengend Snippet: CSF1 and IL34 protein increase throughout development and display distinct spatiotemporal transcriptional expression patterns. (A,B) IL34 (A) and CSF1 (B) protein levels in whole-brain homogenates as measured by ELISA. (C) Representative brain microscopy image with regions of interest outlined in red. Scale bar: 100 µm. (D,F) Representative images of Il34 transcript visualized with RNAscope™ in CA1 (D) and somatosensory cortex (F). Scale bars: 40 µm. (E,G) Plots of the mean percentage of total cells of CA1 (E) and somatosensory cortex (G) that are Il34 + across development. (H,J) Representative images of Csf1 transcript visualized with RNAscope™ in CA1 (H) and somatosensory cortex (J). Scale bars: 40 µm. (I,K) Plots of the mean percentage of total cells of CA1 (I) and somatosensory cortex (K) that are Csf1 + across development. (L) Density maps depicting the spatiotemporal expression patterns of Il34 and Csf1 transcripts in RNAscope™ across development. Warmer colours indicate greater, while cooler colours indicate lower, transcript density. Bars represent mean±s.e.m.; A,B: n =5 mice per time point; E: n =3-5 mice per time point, G: n =4-5 mice per time point, I,K: n =2-3 mice per time point. * P <0.05, **** P <0.0001 (one-way ANOVA, Tukey post-hoc).

Article Snippet: Both IL34 (R&D Systems, M3400) and CSF1 (R&D Systems, MMC00B) ELISAs were conducted as per the manufacturer's instructions.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Microscopy, RNAscope

Journal: Development (Cambridge, England)

Article Title: CSF1R ligands promote microglial proliferation but are not the sole regulators of developmental microglial proliferation

doi: 10.1242/dev.204610

Figure Lengend Snippet: CSF1R signalling is largely restricted to microglia and border-associated macrophages in the P7 hippocampus. (A) UMAP plot depicting the cellular populations of the P7 hippocampus. Dots represent individual cells clustered based on transcriptional similarity, while colours indicate distinct cell lineages or cellular states based on gene expression patterns. (B) UMAP plot depicting expression of Csf1r in the P7 hippocampus. (C) UMAP plot depicting expression of Csf1 in the P7 hippocampus. (D) UMAP plot depicting expression of Il34 in the P7 hippocampus. (E) Dot plot depicting the cell population expression levels of Csf1r , Csf1 and Il34 in the P7 hippocampus. (F) CellChat plot predicting the dominant sender populations that secrete CSF1 and IL34 and dominant receiver populations that receive signalling via CSF1R in the P7 hippocampus. (G) Violin plot depicting the expression of microglial Csf1r across the mouse lifespan. (H) UMAP plot depicting inhibitory neurons, excitatory neurons and non-neuronal cells of the adult hippocampus. Dots represent individual cells clustered based on transcriptional similarity, while colours indicate distinct cell lineages or cellular states based on gene expression patterns. (I) UMAP plot depicting expression of Il34 in the adult hippocampus. (J) Violin plot comparing the expression level of Il34 between inhibitory neurons, excitatory neurons and non-neuronal cells of the adult hippocampus. Datasets for A-F: ; G: ; H-J: . G,J: **** P <0.0001 (one-way ANOVA, Tukey post-hoc).

Article Snippet: Both IL34 (R&D Systems, M3400) and CSF1 (R&D Systems, MMC00B) ELISAs were conducted as per the manufacturer's instructions.

Techniques: Gene Expression, Expressing

Journal: Development (Cambridge, England)

Article Title: CSF1R ligands promote microglial proliferation but are not the sole regulators of developmental microglial proliferation

doi: 10.1242/dev.204610

Figure Lengend Snippet: Unaltered Il34 and Csf1 levels of IL1α and IL1β knockout mice are insufficient to explain aberrant developmental microglial proliferation. (A) Representative whole brain microscopy image with analysed regions of interest outlined in red. (B) Representative images of IBA1 + microglia (red) and KI67 + proliferative cells (white) in the P10 hippocampus and cortex of IL1α and IL1β knockout mice. Scale bars: 40 µm. (C,D) Plots of the mean percentage of hippocampal microglia (C) and somatosensory cortex microglia (D) that are proliferating in wild-type (WT), IL1α and IL1β knockout mice across development. (E,F) Plots of mean hippocampal microglial densities (E) and mean somatosensory cortex microglial densities (F) in WT, IL1α and IL1β knockout mice. (G,H) Plots of the mean percentage of total cells of CA1 that are Il34 + (G) or Csf1 + (H) at P10 in WT, IL1α and IL1β knockout mice. (I,J) Plots of the mean percentage of total cells of somatosensory cortex that are Il34 + (I) or Csf1 + (J) at P10 in WT, IL1α and IL1β knockout mice. Bars represent mean±s.e.m.; C-F: n =6-8 mice per time point; G,I: n =5 mice per genotype; H,J: n =3-5 mice per genotype. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (C-F: two-way ANOVA, Tukey post-hoc; G-J: one-way ANOVA, Tukey post-hoc).

Article Snippet: Both IL34 (R&D Systems, M3400) and CSF1 (R&D Systems, MMC00B) ELISAs were conducted as per the manufacturer's instructions.

Techniques: Knock-Out, Microscopy

( A – C ) Analysis of IL-1β, IL-6, and TNF-α in pleural lavage fluid measured by ELISA at indicated time points after administration of HAM or talc ( n = 4 mice per group) ( , A–C). ( D and E ) IL-1β and CRP in serum measured by ELISA at indicated time points after administration of HAM or talc ( n = 4 mice per group) ( , D and E). ( F ) Residual inflammation on FDG-PET at 3 months after administration of HAM or talc (red arrows). ( G ) The volume voxels with standardized uptake value (SUV) > 1.5 is shown ( n = 3). Data are shown as mean ± SD. * P < 0.05.

Journal: JCI Insight

Article Title: Hydroxyapatite microspheres induce durable pleurodesis and are rapidly cleared by pleural osteoclasts

doi: 10.1172/jci.insight.192981

Figure Lengend Snippet: ( A – C ) Analysis of IL-1β, IL-6, and TNF-α in pleural lavage fluid measured by ELISA at indicated time points after administration of HAM or talc ( n = 4 mice per group) ( , A–C). ( D and E ) IL-1β and CRP in serum measured by ELISA at indicated time points after administration of HAM or talc ( n = 4 mice per group) ( , D and E). ( F ) Residual inflammation on FDG-PET at 3 months after administration of HAM or talc (red arrows). ( G ) The volume voxels with standardized uptake value (SUV) > 1.5 is shown ( n = 3). Data are shown as mean ± SD. * P < 0.05.

Article Snippet: The levels of analytes in mouse pleural lavage fluid and serum were measured by ELISA as follows: TRAP5b, Mouse TRAPTM (TRAP5b) ELISA Kit (Immunodiagnostic Systems, United Kingdom); IL-1β, Mouse IL-1β DuoSet ELISA Kit (R&D Systems Inc); CRP, Mouse C-Reactive Protein DuoSet ELISA Kit (R&D Systems Inc); RANKL, Mouse TRANCE/RANKL/TNFSF11 Quantikine ELISA Kit (R&D Systems Inc); OPG, Mouse Osteoprotegerin/TNFRSF11B DuoSet ELISA Kit (R&D Systems Inc); M-CSF, Mouse M-CSF DuoSet ELISA Kit (R&D Systems Inc) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay

( A and B ) qPCR for osteoclastogenic genes Tnfsf11 (RANKL), Tnfrsf11a (RANK), Tnfrsf11b (OPG), Csf1 (M-CSF), and Csf1r (M-CSFR), in pleural lavage cells collected at indicated time points after intrapleural instillation of HAM (blue) ( A ) or talc (red) ( B ) ( n = 4 mice per group). ELISA of RANKL, OPG, and M-CSF proteins in pleural lavage fluid over time after instillation of HAM (blue) ( C ) or talc (red) ( D ). Data are shown as mean ± SD. Comparisons were by unpaired t test for 2 groups and by 1-way ANOVA followed by Tukey’s multiple-comparison test. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Journal: JCI Insight

Article Title: Hydroxyapatite microspheres induce durable pleurodesis and are rapidly cleared by pleural osteoclasts

doi: 10.1172/jci.insight.192981

Figure Lengend Snippet: ( A and B ) qPCR for osteoclastogenic genes Tnfsf11 (RANKL), Tnfrsf11a (RANK), Tnfrsf11b (OPG), Csf1 (M-CSF), and Csf1r (M-CSFR), in pleural lavage cells collected at indicated time points after intrapleural instillation of HAM (blue) ( A ) or talc (red) ( B ) ( n = 4 mice per group). ELISA of RANKL, OPG, and M-CSF proteins in pleural lavage fluid over time after instillation of HAM (blue) ( C ) or talc (red) ( D ). Data are shown as mean ± SD. Comparisons were by unpaired t test for 2 groups and by 1-way ANOVA followed by Tukey’s multiple-comparison test. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Techniques: Enzyme-linked Immunosorbent Assay, Comparison

( A and B ) qPCR for osteoclast genes Acp5 (TRAP), Ctsk , Atp6v0d2 , Mmp9 , Itgb3 (Integrin β3) and Calcr (Calcitonin receptor), and Oscar (Osteoclast-associated immunoglobulin-like receptor) in pleural lavage cells collected at the indicated time points after intrapleural instillation of HAM (blue) ( A ) or talc (red) ( B ). ( C ) Pleural multinucleated giant cells at 14 days after intrapleural challenge of HAM or talc stained with Giemsa, or for TRAP, CALCR, and CTSK. Scale bars: 20 μm. ( D ) TRAP5b, quantified by ELISA, in pleural lavage fluid from control, HAM- or talc-treated mice collected at 14 days after challenge ( n = 3–4 mice per group). Data are shown as mean ± SD. Comparisons were by unpaired t test for 2 groups and by 1-way ANOVA followed by Tukey’s multiple-comparison test. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Journal: JCI Insight

Article Title: Hydroxyapatite microspheres induce durable pleurodesis and are rapidly cleared by pleural osteoclasts

doi: 10.1172/jci.insight.192981

Figure Lengend Snippet: ( A and B ) qPCR for osteoclast genes Acp5 (TRAP), Ctsk , Atp6v0d2 , Mmp9 , Itgb3 (Integrin β3) and Calcr (Calcitonin receptor), and Oscar (Osteoclast-associated immunoglobulin-like receptor) in pleural lavage cells collected at the indicated time points after intrapleural instillation of HAM (blue) ( A ) or talc (red) ( B ). ( C ) Pleural multinucleated giant cells at 14 days after intrapleural challenge of HAM or talc stained with Giemsa, or for TRAP, CALCR, and CTSK. Scale bars: 20 μm. ( D ) TRAP5b, quantified by ELISA, in pleural lavage fluid from control, HAM- or talc-treated mice collected at 14 days after challenge ( n = 3–4 mice per group). Data are shown as mean ± SD. Comparisons were by unpaired t test for 2 groups and by 1-way ANOVA followed by Tukey’s multiple-comparison test. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Control, Comparison

Review

csf1 (R&D Systems)

Structured Review

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1) Product Images from "CSF1R ligands promote microglial proliferation but are not the sole regulators of developmental microglial proliferation"

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